BioDieselNow - Renewable biodiesel fuel

Ciao,

di dove sei?

Anche io sto lavorando nella produzione di biodiesel da alghe. Sto facendo un dottorato at Univesity of Texas at austin,

Ciao

fedenoris:

Ciao,

di dove sei?

Anche io sto lavorando nella produzione di biodiesel da alghe. Sto facendo un dottorato at Univesity of Texas at austin,

 

Ciao

Rather rough translation (Italian to English) provided by  AltaVista Babel (http://babelfish.altavista.com/tr)

 "Hello, of where six? Also I am working in the production of biodiesel from alghe. I am making a doctorate at Univesity of Texas at austin, Hello"
 

Many many thanks.

 :)

So far I'm still not very sure about what are those tiny little algae in my culture. They look very different from the stock culture.

Hello.

I am working with a group at my school to produce a biodiesel. We are using B. braunii, which we bought from UTEX. It came in modified bold 3N medium, however, it has solidified into a gel, and we don't know how to get it out. Does anyone have any suggestions?

thanks
 

Hi, Just use a microbial standard swab (it is like a fine wire with a loop at the end). Sterilize the loop (burn it with bunsen burner until it turn red), cool it down, and insert it into the culture tube. Take one or two colonies out with the loop and swirl it in the culture medium that you prepared. If you have laminar flow hood, it is better to do these steps inside the hood :)

Hope this helps. All the best.

I'm certainly no algae expert but..

 The little "oil gobbules" or  what you see as little spheres inside the spheres are actually baby algae.  (at least i'm 90% sure they are)..

 The tiny little things outside the cell walls are just baby algae.

If anyone needs their stuff checked out, I bought a good microscope to check this stuff out..  I'm growing my own and still separating and isolating strains.

Ya, it sounds logical since when i isolate them from the tube, the growing condition in the agar medium is so limited that the bb have to form spores for the survival inside the tube. When it is transferred into the liquid medium, it allows the algae to divide mitotically where energy and nutrients are abundant.

Besides, in my lab, i don't think there is other source of foreign algae that can contaminate my culture. I've tried out many times. The results are all the same. Now I would like to reverse the experiment. I'll isolate the baby bb and grow them on agar until the nutrient on the agar is depleted, and see whether they will turn into those bigger oval shape bb.

(I read some books it says algae undergo sexual reproduction only there is sufficient nutrient supply. It contradict with the statement above, I wonder).

Some algae change their phenotype when the environment changes or in alternating generations.  Based on what I've been able to gather, it's not that well understood.   Probably the best way is with a DNA test.  You also might try 'shocking' it with nitrogen deprivation. (i.e. putting it in a low nitrogen nutrient solution for an indeterminate amount of time).  It might make sense to shock it with low phosphates in a different experiment, etc

I still can't solve this problem. What are those smaller thingy....

Hi Liberty1

I think I can ship 100 ml of concentrated BB to anywhere in USA for $50/- . This should be enough to innoculate b/w

1litre to 5 litres of culture solution (F/2 guillards)

compared to UTEX barely can see for $70/-

Kindly let me know if you think this is a reasonable price....This is my first time and I am not trying to make a lot of profit

but be able to provide to all seekers at reasonable costs...

Thanks