Hi EoaPond,
I am not sure exactly why everyone is pushing salt water algae if you are in an area where you have unlimited freshwater. It sounds like you may live in a rural area so does that mean you get your water from a well? Where I am working we have streams set up using multiple types of water. There are three types of freshwater streams being simulated: 1.well water 2. enriched well water (N and P added) 3. wastewater effluent from about a half mile-mile down the channel from a wastewater treatment plant. The streams with enriched well water are doing very well as are the wastewater streams. The straight well water is supporting growth but not as much as the other streams.(expected)
If you are near farms, you could get nutrients easily in bulk form, cant you?
What is the temperature like in the indoor place you are growing the algae? I have some tanks set up in a greenhouse that gets extremely warm during the day. My cultures at times have been as high as 40 degrees Celsius which sounds like a very bad thing, but it is actually making one of my species thrive. In one tank I have both Scenedesmus quadricauda and Neochloris oleoabundans and I have found that the oleoabundans has taken off and outcompeted the Scen. by far. The Neochloris was isolated from the desert in Saudi Arabia so I think it would make sense that oleoabundans thrives in the hot temperatures. Although this change has occured in the ratio of species in my tank, the Scenedesmus is still surviving in lower numbers, which could indicate a more stable system.
I would suggest to look into the use of multiple species at once. That way, if you have fluctuations in temperature depending on season, you can always have something in your tank that can thrive. Many species do very well in an all-purpose medium such as Bold. I would consider the simplified way marc from ecogenics feeds his algae. If you search through old posts(or if he would post it again), he explained a very simple method using soil water for most of the required micro and macro nutrients along with simple fertilizer.
Bacteria and fungus can also pose a slight problem. There is no doubt going to be bacteria in your cultures so you should think about how that might affect what happens within your tanks. For me it hasn't been a serious problem since the algae grow in such high numbers and there could even be benefits to bacteria. Algae thrive in nature with bacteria so I don't see why it should be a major problem in a tank.
I looked into pressing algae for a long time and searched through a great deal of literature. I could not find conclusive evidence anywhere that showed someone has successfully pressed oil out of their algae. Don't be discouraged though, because I also didn't find anyone who actually tried. There are some people in the other BDnow forums that discuss the use of presses for their various feedstocks. I found that some of those people were very willing to test some algae in their presses. I would recommend trying some different presses before investing in one to figure out what would work the best. It also looked promising that a press could be modified to use for algae, though it would take permanent changes that wouldn't be guaranteed to work(on expensive machinery).
As far as oil content goes, I have read lipid %'s for my 2 species that claimed lipids as high as 50 or 60%. After a few small-scale chemical extractions, the percentages I observed were 10.5% for Neochloris and 12.3% for Scenedesmus. I harvested these straight from my growth tanks without any stresses being applied to increase the oil content. I am trying that now so I will post when I get some more results. Considering that the cells I harvested were 1/10 lipid without stress, I am optimistic that the percentage will be at least in the 30-40% range. You should look into literature that discusses the effect of stressing algae. I think this is an important step of the process because most algae stores energy as carbohydrate. You want the type of lipids used in energy storage that are nonpolar, which are produced when the algae is stressed, most often by lack of Nitrogen. This is how you can get the algae to produce TAGs which is the oil that can be converted into biodiesel. It might involve having a second tank with just plain water to give the algae one or two days to produce the lipids you want.
Your idea sounds great to use the pressed mash for fuel. That is a good way to take advantage of the inefficiency of the cold press that is known to leave behind oils. I bet those will help your pellets burn nice and hot.
What type of stove to you use to heat your house? My parents installed a Tulikivi last year in their home and I cannot believe how efficient it is. They can heat nearly the whole house by only burning 2 hot fires a day because of the heat-retaining and radiating properties of the soapstone. Is this much different from your pellet-burning heater?
One last thing: Why is everyone pushing seawater so much? wouldn't that add extra cost and complication to his operation? I am aware of some benefits with an outdoor system, but does it really make much of a difference if the algae is being grown indoors?