respected sir, I'm BHUVANESH. N student of Tamil Nadu Agricultural University , studying 2nd M.Sc(agricultural microbiology) , coimbatore, Tamil nadu, India. Im doing my research on " ALKANE SYNTHESIS BY Botryococcus". Im collecting samples from different locations of tamilnadu and my research...

I measured the co2 in my incubating chamber, it's 2726 ppm vs a normal atmosphere of 350 to 450. Quite a difference, almost5.5x normal atmosphere. Co2 is produced bu baking soda and vinegar mix once a day, with stirring done twice a day. It is having a positive result. Chamber temp is 91 deg F, same...

I am using microscopic algae, and I've found the method for extracting the oil from the algae (AOAC 996.06). However, I'm not sure how to get the algae ready for GC. Will I be filtering it off and freezing it? Is GCMS the best method? or is gravimetrical a better way to go?